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In vitro Vasorelaxant Effect of Artemisia herba alba Asso. in Spontaneously Hypertensive Rats

[ Vol. 14 , Issue. 3 ]

Author(s):

Zeggwagh Naoufel, Morad Hebi, Mohammed Ajebli, Jean B. Michel and Mohamed Eddouks   Pages 190 - 196 ( 7 )

Abstract:


Background: The present study aims to evaluate the in vitro vasorelaxant effect of Artemisia herba alba (Ah) aqueous extract.

Method: Aortic rings were isolated from spontaneously hypertensive rats and incubated in aqueous Ah extract at the following doses: 3, 5, 10 and 20 mg/ml. Incubation of aqueous Ah extract for 30 minutes produced a significant shift of the dose response curve to Norepinephrine (NE) (10-8 to 10-5 M) (p<0.001).

Results: Endothelium ablation significantly reduced the vasorelaxant effect of aqueous Ah extract (p<0.001). In addition, inhibition of vascular nitric oxide by Nω-Nitro-L-Arginine Methyl (LNAME) produced a significant reduction in the vasorelaxant effect of aqueous Ah extract (p<0.001).

Conclusion: We conclude that aqueous Ah extract at a dose of 20 mg/ml possess an in vitro vasorelaxant effect which seems to be dependent on the endothelium vasorelaxant factors involving nitric oxide synthesis.

Keywords:

Aortic rings, Artemisia herba alba, endothelium vasorelaxant factors, ethnophamacology, hypertension, nitric oxide synthesis.

Affiliation:

High School of Nursing and Health Techniques of Rabat, Avenue Hassan II Km 4,5 Rabat,, Laboratory of Physiology and Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, B.P. 21, Errachidia, 52000,, Laboratory of Physiology and Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, B.P. 21, Errachidia, 52000,, U1148 INSERM, Faculty of Medicine Xavier Bichat, Paris,, Laboratory of Physiology and Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 21, Errachidia, 52000

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